Effects of different lactic acid bacteria on the physicochemical properties, functional characteristics and metabolic characteristics of fermented hawthorn juice

Lactic acid bacteria (LAB) fermentation enhances the flavour and functionality of juice substrates; however, research on hawthorn juice is limited. We hypothesize that due to strain specificity, the changes in hawthorn juice after fermentation with different LAB may vary. After selecting LAB strains based on pH and sensory evaluation, the physicochemical properties and anti-inflammatory potential in a lipopolysaccharide-induced RAW 264.7 macrophage model were analysed in vitro. Non-targeted metabolomics revealed fermentation-driven metabolic changes. All strains exhibited increased total acidity and decreased reducing sugar and flavonoid contents. In particular, the Lactobacillus plantarum SC-1.3 and FWDG (strain preservation number) strains suppressed the pro-inflammatory cytokines interleukin-6 and tumour necrosis factor-α, with FWDG exhibiting the strongest effect. Moreover, fermentation resulted in the enrichment of bioactive metabolites, including prunetin and glycitein, which are unique to FWDG. The results provided a basis for the industrialization of hawthorn juice as a dietary product.