Key enzymatic activities and metabolic pathway dynamics in acidogenic fermentation of food waste: Impact of pH and organic loading rate

Acidogenic fermentation was an effective technology to recover volatile fatty acids (VFAs) ethanol and lactic acid from food wastes (FW) as bioresources. However, the impact of process controls on key functional enzymes and metabolic pathways has been inadequately understood. In this study, the metabolite distribution, key functional enzymes and metabolic pathways were completely elucidated using 16S rRNA gene high-throughput sequencing combined with PICRUSt2. Results demonstrated pH significantly affected fermentation types by influencing key enzyme activities, while organic loading rate (OLR) primarily affected the yield without altering metabolic pathway. The maximum VFAs production was achieved at pH 6.0 and OLR of 15.0 g-VS/L/d as a result of Glycolysis and Pyruvate Metabolism were enhanced. Meanwhile, butyric acid was always dominant product, attributed to the enhanced activity of butyryl-CoA dehydrogenasedue. Furthermore, Lactobacillus enrichment and lactate dehydrogenase upregulation promoted lactate-type fermentation under without pH control (3.8), resulting in an average yield of lactic acid was 7.84 g/L. When the pH was raised from 3.8 to 5.0,downregulation of lactate dehydrogenase and upregulation of acetate kinase shifted the fermentation to acetate-type. This study provides a deeper understanding of how does process controls influence the metabolic pathways and key functional enzymes.