Objective: To investigate the expression of miR-383-5p in newly diagnosed multiple myeloma (MM) and its correlation with clinical features and prognosis.
Methods: 115 MM patients diagnosed and treated in the Department of Hematology, the First Affiliated Hospital of Bengbu Medical University from January 2013 to January 2017 and 115 non-tumor controls were enrolled in this study. Clinical characteristics, pathological data and therapeutic responses of the patients were collected. The expression of miR-383-5p in MM patients and non-tumor controls was detected by RT-qPCR, and the relationship between the expression level of miR-383-5p and the clinical characteristics and prognosis of MM patients was further analyzed.
Results: The results of RT-qPCR showed that the relative expression levels of miR-383-5p in bone marrow tissues of 115 non-tumor controls and 115 MM patients were 1.89±0.11 and 1.48±0.13, respectively, and the difference was statistically significant (P < 0.05). Chi-square analysis showed that the expression of miR-383-5p was correlated with bone injury, β2-microglobulin and globulin (all P < 0.001), but not with other factors including age, sex, hemoglobin and light chain. Univariate and multivariate Cox regression analysis indicated that low expression of miR-383-5p was an independent risk factor for poor OS in MM patients (P < 0.001).
Conclusion: The expression of miR-383-5p is down-regulated in MM patients, and low expression of miR-383-5p is a risk factor for poor prognosis and can be used as a new prognostic biomarker.
题目: 多发性骨髓瘤细胞表达miR-383-5p 的研究及临床意义.
目的: 探讨miR-383-5p 在初治多发性骨髓瘤（MM）中的表达及其与临床特征及预后的相关性。.
方法: 回顾性收集2013年1月至2017年1月蚌埠医科大学第一附属医院血液科确诊及治疗的初治MM患者及非肿瘤对照者各115例，收集患者临床特征、病理资料及治疗反应，通过RT-qPCR检测miR-383-5p 在MM患者及非肿瘤对照者中的表达情况，进一步分析miR-383-5p 表达量与MM患者临床特征及预后的关系。.
结果: RT-qPCR结果显示，115例非肿瘤对照者骨髓组织与115例MM患者骨髓组织中miR-383-5p 相对表达量分别为1.89±0.11与1.48±0.13，比较差异有统计学意义（P < 0.05）。卡方检验结果提示，miR-383-5p 表达与骨损伤、β2-微球蛋白和球蛋白相关（均P < 0.001），但与其他因素包括年龄、性别、血红蛋白和轻链不相关。单变量与多变量Cox回归分析表明，低表达miR-383-5p 是MM患者OS不良的独立危险因素（P < 0.001）。.
结论: miR-383-5p 在MM患者中表达下调，且低表达miR-383-5p 是预后不良的危险因素，可作为新的预后生物标志物。.
Keywords:
multiple myeloma;