Objective: To investigate the role of matrix metalloproteinase-13 (MMP-13 ) in the development, diagnosis and prognosis of multiple myeloma (MM).
Methods: Blood samples of 57 MM patients and 45 normal controls were collected, and real-time PCR was performed to detect the MMP-13 mRNA expression level in the study subjects, and the difference of MMP-13 mRNA level between MM patients and normal controls was compared. The correlations of MMP-13 with MM bone disease and its severity, ISS stage, DS stage, and treatment efficacy were analyzed.
Results: The MMP-13 mRNA in patients with MM was significantly higher than that in normal controls (P < 0.05). The MMP-13 mRNA in MM patients with bone disease was significantly higher than that in patients without bone disease, and the more severe the bone disease, the more obvious the increase in MMP-13 mRNA (P < 0.05). The MMP-13 gene expression level was also significantly different between ISS and DS stage Ⅰand stage Ⅲ patients (P < 0.05), and the MMP-13 mRNA level was significantly decreased after treatment (P < 0.05).
Conclusion: The MMP-13 mRNA expression level is related to the occurrence and development of MM, and it has certain guiding significance in disease diagnosis and prognosis evaluation.
题目: 基质金属蛋白酶-13在多发性骨髓瘤中的作用研究.
目的: 探讨基质金属蛋白酶-13（MMP-13 ）在多发性骨髓瘤（MM）疾病发生发展及病情判断、预后评估等方面的作用。.
方法: 收集MM患者57例、正常对照人群45例，采用实时荧光定量PCR检测研究对象MMP-13 基因mRNA表达水平，比较MM患者组和正常对照组MMP-13 mRNA表达水平的差异，分析MMP-13 与MM骨病及其严重程度、ISS分期、DS分期及治疗效果的关系。.
结果: MM患者MMP-13 mRNA表达水平较正常对照组显著升高（P < 0.05）；有骨病的MM患者较无骨病的患者MMP-13 mRNA明显升高，且骨病越严重升高越明显（P < 0.05）；ISS分期及DS分期Ⅰ期与Ⅲ期患者之间MMP-13 mRNA差异也有统计学意义（P < 0.05）；患者治疗后较治疗前MMP-13 明显下降（P < 0.05）。.
结论: MMP-13 基因mRNA表达水平与MM发生发展相关，其表达水平在病情判断、预后评估等方面具有一定的指导意义。.
Keywords: matrix metalloproteinase-13; multiple myeloma; myeloma bone disease; stage; treatment efficacy.