Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae)

Hao He; Yongxin Zhao; Dengyu Zheng; Huiyun Kuang; Fei Shen; Kang He; Haiyan Zhang; Changjiang Zhao; Lu Jiang; Da Xiao; Su Wang; Zhenying Wang; Shuai Zhan; Jianhua Wei; Xiaozeng Yang; Zhongyi Wu; Chun Zhang

doi:10.1038/s41597-024-04360-3

Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae)

Sci Data. 2025 Jan 2;12(1):1. doi: 10.1038/s41597-024-04360-3.

Authors

Hao He^#¹, Yongxin Zhao^#¹, Dengyu Zheng^#¹, Huiyun Kuang^#², Fei Shen¹, Kang He³, Haiyan Zhang⁴, Changjiang Zhao⁴, Lu Jiang⁵, Da Xiao⁶, Su Wang⁶, Zhenying Wang⁷, Shuai Zhan⁸, Jianhua Wei⁹, Xiaozeng Yang^{10

11}, Zhongyi Wu¹², Chun Zhang¹³

Affiliations

¹ Institute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
² Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.
³ State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects & Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
⁴ College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang, China.
⁵ Key Laboratory of Economic and Applied Entomology of Liaoning Province, College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China.
⁶ Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Science, Beijing, 100097, China.
⁷ Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
⁸ Key Laboratory of Plant Design, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
⁹ Institute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. [email protected].
¹⁰ Institute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. [email protected].
¹¹ State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. [email protected].
¹² Institute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. [email protected].
¹³ Institute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. [email protected].

^# Contributed equally.

Abstract

Monolepta hieroglyphica, in view of its wide-ranging host and highly polyphagous characteristics, has become an important agricultural pest in East and Southeast Asian countries. To better understand its biology and develop control strategies, we present a high-quality chromosome-level genome assembly of M. hieroglyphica, with contig N50 of 18.62 Mb and scaffold N50 of 241.916 Mb. Using 156x Hi-C data, we anchored a total length of 2,313 Mb (91.9% of the genome) to nine chromosomes. We predicted 46,561 genes using an integrated approach. The completeness of the final reported genome and proteome were assessed by BUSCO and show high values of 99.4% and 95.2%, respectively. The high-quality chromosome-level genome assembly of M. hieroglyphica will be a useful molecular resource for the communities of both beetle and crop protection.

Publication types

Dataset

MeSH terms

Animals
Chromosomes, Insect / genetics
Coleoptera* / genetics
Genome, Insect*