Background: Cerebrovascular injury is a common pathological feature of a spectrum of neurological disorders, including traumatic brain injury (TBI), stroke, Alzheimer's disease (AD), and aging. Vascular manifestations among these conditions are similar indeed, including the breakdown of the blood-brain barrier (BBB). However, whether there is a unique molecular mechanism underlying the vascular changes among these conditions remains elusive.
Method: We performed single-cell RNA (scRNA-seq) sequencing and obtained 29,114 high-quality cells combined from ipsilateral hemispheres of WT C57BL/6J Sham mice and mTBI mice at day 1, 3 and 7.
Result: Joint clustering of all groups revealed 7 cell populations using their specific genetic markers, including vEC, vcapEC, capEC, acapEC, aEC. Next, we identified differential expression genes between Sham and day 1 or day 3 or day 7 post-mTBI within each endothelial cell cluster.
Conclusion: Based on transcriptomic analysis on cerebrovascular scRNA-seq datasets, we identified a common molecular signature between mTBI and aging vasculature, involving a novel transmembrane protein gene Tmem252. Tmem252 upregulation in brain endothelial cells may represent a shared endophenotype of microvascular injuries in different pathological conditions, and therapeutically targeted for treating BBB breakdown and vascular dysfunctions.
© 2024 The Alzheimer's Association. Alzheimer's & Dementia published by Wiley Periodicals LLC on behalf of Alzheimer's Association.