Electrochemical impedance spectroscopy (EIS) serves as a non-invasive technique for assessing cell status, while mechanical stretching plays a pivotal role in stimulating cells to emulate their natural environment. Integrating these two domains enables the concurrent application of mechanical stimulation and EIS in a stretchable cell culture system. However, challenges arise from the difficulty in creating a durable and stable stretchable impedance electrode array. To overcome this limitation, we have developed a cell culture system integrated with a stretchable impedance electrode array (SIEA). This design enabled impedance monitoring during cell proliferation under mechanical stimulation over a long period of time due to the high mechanical durability and electrochemical stability of the SIEA. Our evaluation also involved testing the cytotoxicity of doxorubicin on H9C2 cells directly on the SIEA. The results underscore the significant potential of our SIEA device as an effective tool for monitoring cells subjected to mechanical stimulation and as a platform for drug toxicity testing.