Abstract
Cordycepin, a key bioactive compound produced by Cordyceps militaris, faces the challenge of low productivity for commercial use. In this study, alanine supplementation in Cordyceps militaris boosted cordycepin production, peaking at 3 mg/g with 12 g/L concentration. Transcriptome analysis revealed 1711 differentially expressed genes, Pathway analysis indicates that protein processing in the endoplasmic reticulum was the most affected pathway. In addition, the transcriptome showed that adenylosuccinate lyase is essential for the synthesis of cordycepin. The modulation of four genes (Cns1-4) points to a regulatory mechanism that could increase cordycepin biosynthesis, offering a strategy to overcome low productivity for commercial applications.
MeSH terms
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Cordyceps* / chemistry
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Cordyceps* / genetics
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Cordyceps* / metabolism
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Deoxyadenosines* / biosynthesis
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Deoxyadenosines* / metabolism
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Fungal Proteins* / genetics
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Fungal Proteins* / metabolism
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Gene Expression Regulation, Fungal / drug effects
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Up-Regulation / drug effects
Substances
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cordycepin
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Deoxyadenosines
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Fungal Proteins
Grants and funding
This work was supported by the the National Natural Science Foundation of China (grant number 32260017, 31960193); Double Thousand Plan of Jiangxi Province (grant number jxsq 2019201011); the Natural Science Foundation of Jiangxi Province (grant number 20202ACBL206029); Jiangxi Provincial Natural Science Foundation (grant number 20242BAB25334) and youth talent support program of Jiangxi Science and Technology Normal University (grant number 2022QNBJRC005).