Inoculation with effective microorganisms agent enhanced fungal diversity in the secondary fermentation process

Xiayan Liu; Lingyu Kong; Lihong Tong; G S K Zackariah; Rongsheng Zhu; Zhaojun Li; Yizhong Lv

doi:10.1016/j.jenvman.2024.123985

Inoculation with effective microorganisms agent enhanced fungal diversity in the secondary fermentation process

J Environ Manage. 2025 Jan 2:373:123985. doi: 10.1016/j.jenvman.2024.123985. Online ahead of print.

Authors

Xiayan Liu¹, Lingyu Kong², Lihong Tong³, G S K Zackariah⁴, Rongsheng Zhu⁵, Zhaojun Li⁶, Yizhong Lv²

Affiliations

¹ Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong, China; Department of Soil and Water Sciences, College of Land Science and Technology, China Agricultural University, Beijing, 100193, China.
² Department of Soil and Water Sciences, College of Land Science and Technology, China Agricultural University, Beijing, 100193, China.
³ State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, Qinghai, China.
⁴ State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
⁵ Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong, China.
⁶ Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong, China. Electronic address: [email protected].

PMID: 39752954
DOI: 10.1016/j.jenvman.2024.123985

Abstract

Microbial inoculations have emerged as a key approach to address the low natural microbial activity of traditional composting technologies. It is crucial for successfully promoting manure composting to understand the influences of microbial inoculations on fungal communities and its mechanisms. To investigate the effects of microbial inoculation on diversity characteristics, tropic mode, and co-occurrence network of fungal communities during composting, an aerobic composting experiment of chicken manure inoculated with microbial agents was performed. The results showed that microbial inoculations enhanced fungal richness and diversity during the secondary fermentation, promoted beneficial fungi, and restrained pathogenic microbes. Microbial inoculation facilitated saprophytic fungi and symbiotic fungi, augmented fungal network complexity and cooperation during the first fermentation, concurrently impeding fungal network complexity and cooperation during the secondary fermentation. These results provide technical guidance for composting process optimization and compost product quality improving, which was beneficial to promote soil quality and mitigating agricultural non-point source pollution.

Keywords: Co-occurrence network; Fungal community; Microbial inoculations; Tropic mode.