A colorimetric nano-enzyme assay with Ni@Pt nanoparticles as signal labels for rapid and sensitive detection of exosomal Aβ42 in plasma

Junli Zhang; Yanyan Zhao; Haiying Hu; Min Lv; Hongling Zhang

doi:10.1007/s00604-024-06862-8

A colorimetric nano-enzyme assay with Ni@Pt nanoparticles as signal labels for rapid and sensitive detection of exosomal Aβ42 in plasma

Mikrochim Acta. 2025 Jan 3;192(1):53. doi: 10.1007/s00604-024-06862-8.

Authors

Junli Zhang^{1

2}, Yanyan Zhao¹, Haiying Hu¹, Min Lv¹, Hongling Zhang^{3

4}

Affiliations

¹ School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, China.
² Henan Key Laboratory of Nanomedicine for Targeting Diagnosis and Treatment, Zhengzhou, 450001, China.
³ School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, China. [email protected].
⁴ Henan Key Laboratory of Nanomedicine for Targeting Diagnosis and Treatment, Zhengzhou, 450001, China. [email protected].

PMID: 39753867
DOI: 10.1007/s00604-024-06862-8

Abstract

A nano-enzyme sandwich assay (SWzyme assay), a colorimetric system based on a biochip and inorganic nano-enzyme for rapid and simple determination of exosomal Aβ42 in plasma is proposed. Anti-CD63 antibody-modified biochips were prepared for plasma exosome capture and synthesized highly catalytic Ni@Pt nanozymes for detecting exosomal Aβ42. The method was able to detect exosomal Aβ42 with a limit of detection (LOD) as low as 4.2×10⁴ particles/mL and a linear range from 10⁴ to 10⁸ particles/mL. By determination of exosomal Aβ42, the SWzyme assay successfully distinguished plasma from Alzheimer's desease (AD) and healthy mice. The SWzyme assay holds promise to serve as diagnostic tools for the early detection of AD and supporting the development of personalized medicine.

Keywords: Alzheimer’s disease; Biochip; Colorimetric; Exosomal Aβ42; Nanozymes.

Publication types

Research Support, Non-U.S. Gov't