Multi-functional electrochemiluminescence biosensor for efficient capture, elimination, and sensitive monitoring of Staphylococcus aureus

Biosens Bioelectron. 2025 Mar 15:272:117112. doi: 10.1016/j.bios.2024.117112. Epub 2024 Dec 29.

Abstract

In this study, we developed a novel strategy for effective bacteria capture, elimination, and detection. The aptamer of Staphylococcus aureus (S. aureus) was immobilized on Fe3O4 NPs and partly hybridized with the T strand, which exhibited good bacterial capture efficiency. Even though high concentration of S. aureus (7.7 × 106 CFU/mL) was introduced, almost all the bacteria were combined on Fe3O4 in 60 min. Meanwhile, efficient amplification strategies include strand-displacement reaction, DNA walker, and essential oil-synthesized Au:Ag NPs were developed for the sensitive measurement of the released T strand during the capture of S. aureus. The green-synthesized Au:Ag NPs can effectively kill S. aureus while also significantly improving the ECL signal (16.7-fold). Owing to the outstanding features of Au:Ag NPs, this novel biosensor displayed a wide linear range and a low detection limit for S. aureus measurement. As a result, this multifunctional technique shows great potential for practical applications in the fields of food safety and the environment.

Keywords: Bacterial removal; Bimetallic nanoparticles; Electrochemiluminescence; Essential oil; SDA.

MeSH terms

  • Aptamers, Nucleotide* / chemistry
  • Biosensing Techniques* / methods
  • Electrochemical Techniques* / methods
  • Gold* / chemistry
  • Humans
  • Limit of Detection*
  • Luminescent Measurements* / methods
  • Metal Nanoparticles* / chemistry
  • Silver / chemistry
  • Staphylococcal Infections / diagnosis
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus* / isolation & purification

Substances

  • Gold
  • Aptamers, Nucleotide
  • Silver