LncRNA MIR210HG promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to PCBP1

Sci Rep. 2025 Jan 6;15(1):871. doi: 10.1038/s41598-025-85321-7.

Abstract

This study aimed to investigate the role of the MIR210 host gene (MIR210HG), a long noncoding RNA (lncRNA), in the proliferation of colon cancer cells and its potential mechanism involving the ferroptosis pathway. We assessed MIR210HG expression in colon cancer cell lines and tissues, and examined the effects of its overexpression and knockdown on cell proliferation. Proteomic analysis was conducted to explore the interaction between MIR210HG and ferroptosis pathway components. The binding of MIR210HG to poly(rC) binding protein 1 (PCBP1) was predicted using catRAPID and confirmed through RNA pull-down and RNA immunoprecipitation (RIP) experiments. MIR210HG was significantly upregulated in colon cancer cells and tissues. Its overexpression promoted, while its knockdown inhibited, colon cancer cell proliferation. MIR210HG was found to be associated with ferroptosis pathway components and to bind to PCBP1, which was experimentally validated. The inhibition of ferroptosis by MIR210HG through PCBP1 binding was confirmed, highlighting its role in promoting cell proliferation. MIR210HG promotes colon cancer cell proliferation by binding to PCBP1 and inhibiting ferroptosis. These findings suggest MIR210HG as a potential therapeutic target for colon cancer.

Keywords: Colon cancer; Ferroptosis; Long noncoding RNA; MIR210HG; PCBP1; Proliferation.

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation*
  • Colonic Neoplasms* / genetics
  • Colonic Neoplasms* / metabolism
  • Colonic Neoplasms* / pathology
  • DNA-Binding Proteins* / genetics
  • DNA-Binding Proteins* / metabolism
  • Ferroptosis* / genetics
  • Gene Expression Regulation, Neoplastic*
  • Heterogeneous-Nuclear Ribonucleoproteins* / genetics
  • Heterogeneous-Nuclear Ribonucleoproteins* / metabolism
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • Protein Binding
  • RNA, Long Noncoding* / genetics
  • RNA, Long Noncoding* / metabolism
  • RNA-Binding Proteins* / genetics
  • RNA-Binding Proteins* / metabolism

Substances

  • RNA, Long Noncoding
  • RNA-Binding Proteins
  • PCBP1 protein, human
  • DNA-Binding Proteins
  • Heterogeneous-Nuclear Ribonucleoproteins
  • MicroRNAs