Mueller-Matrix Interferometric Multifractal Scaling of Optically Anisotropic Architectonics of Diffuse Blood Facies: Fundamental and Applied Aspects

J Biophotonics. 2025 Jan 6:e202400412. doi: 10.1002/jbio.202400412. Online ahead of print.

Abstract

The article describes a technique for digital holographic reconstruction of complex amplitude fields in diffuse blood facies using laser polarization-interference phase scanning to isolate a single scattered component of the object field. This method serves as the basis for developing algorithms for Mueller-matrix reconstruction of linear and circular birefringence parameters in the polycrystalline architectonics of blood facies. Statistical (central moments of the 1st-4th orders) and multifractal analyses (fractal dimension spectra) are applied to study the optical anisotropy maps of polycrystalline networks during blood dehydration. The study explores a practical application in the differential diagnosis of blood loss volume, identifying higher-order central moments (skewness, kurtosis) as sensitive markers. The method achieved a maximum accuracy of 92.9% in differentiating blood loss volume.

Keywords: blood facie; digital holography; interference; multifractal; optical anisotropy; polarization; statistical moments; wavelet analysis.