Tenacibaculum platacis sp. nov., Tenacibaculum vairaonense sp. nov. and Tenacibaculum polynesiense sp. nov. isolated from batfish (Platax orbicularis) in Tahiti Island, French Polynesia

Ten novel Gram-negative, aerobic, non-sporulating, yellow-pigmented rod-shaped bacterial strains motile by gliding were isolated from marine organisms/environments in French Polynesia. Three of them designated as 190524A05c^T, 190524A02b^T and 190130A14a^T were retrieved from orbicular batfish (Platax orbicularis) mucus. Online database comparisons using 16S rRNA amplicons resulted in over 95% similarity to the genus Tenacibaculum. Phylogenetic analyses based on 679 concatenated core protein sequences revealed that strains 190524A05c^T, 190524A02b^T and 190130A14a^T showed the highest similarity to Tenacibaculum skagerrakense DSM 14836^T, Tenacibaculum xiamenense LMG 27422^T and Tenacibaculum holothuriorum S2-2^T, respectively. Digital DNA-DNA hybridization and average nt identity values between strains 190524A05c^T, 190524A02b^T and 190130A14a^T and other type strains were less than 76.25 and 24.1%, respectively. The DNA G+C content was 31.48, 30.66 and 31.98 mol% for strains 190524A05c^T, 190524A02b^T and 190130A14a^T, respectively. Menaquinone-6 was detected as the major isoprenoid quinone in these three strains. The major polar lipids (phosphatidylethanolamine and aminophospholipid) were similar to the chemotaxonomic profile of other species of the genus Tenacibaculum. Strain 190524A05c^T contained summed feature 3 (comprising C_16:1 ω7c and/or iso-C_15:0 2-OH), iso-C_15:1 G, iso-C_15:0 and iso-C_17:0 3-OH as the major cellular fatty acids. Strain 190524A02b^T contained summed feature 3 (comprising C_16:1 ω7c and/or iso-C_15:0 2-OH), iso-C_15:0, iso-C_15:1 G and iso-C_17:0 3-OH as the major cellular fatty acids. Strain 190130A14a^T contained iso-C_15:1 G, summed feature 3 (comprising C_16:1 ω7c and/or iso-C_15:0 2-OH), iso-C_15:0 and iso-C_17:0 3-OH as the major cellular fatty acids. Based on the phenotypic and molecular features, these three strains represent novel species of the genus Tenacibaculum for which the names Tenacibaculum platacis sp. nov., with 190524A05c^T (= CIP 112470^T = DSM 118113^T) as the type strain; Tenacibaculum vairaonense sp. nov., with 190524A02b^T (= CIP 112469^T = DSM 118112^T) as the type strain; and Tenacibaculum polynesiense sp. nov., with 190130A14a^T (= CIP 112468^T = DSM 118111^T) as the type strain, are proposed.