Diterpenoids from the bark of Torreya grandis and their cytotoxicity activity

Yu-Fan Meng; Jing-Jing Yuan; Mao-Sheng Zhang; Da-Le Guo; Pan-Xiang Cao; Jian-Wen Yang; Min-Jian Dong; Jun Dai; Cheng-Xin Sun; Shi-Ji Xiao

doi:10.1016/j.fitote.2025.106376

Diterpenoids from the bark of Torreya grandis and their cytotoxicity activity

Fitoterapia. 2025 Jan 4:106376. doi: 10.1016/j.fitote.2025.106376. Online ahead of print.

Authors

Yu-Fan Meng¹, Jing-Jing Yuan², Mao-Sheng Zhang³, Da-Le Guo⁴, Pan-Xiang Cao⁵, Jian-Wen Yang³, Min-Jian Dong³, Jun Dai⁶, Cheng-Xin Sun⁷, Shi-Ji Xiao⁸

Affiliations

¹ Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi 563000, China; Guilin Hospital of the Second Xiangya Hospital CSU, Guilin 541199, China.
² Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi 563000, China.
³ Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi 563000, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, China.
⁴ State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
⁵ Guizhou Wuchuan Wannianfeng Agricultural Development Co., Ltd., Zunyi 563000, China.
⁶ Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi 563000, China; Laboratory Animal Center, Zunyi Medical University, Zunyi 563000, Guizhou, China. Electronic address: [email protected].
⁷ Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi 563000, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, China. Electronic address: [email protected].
⁸ Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi 563000, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, China. Electronic address: [email protected].

PMID: 39761704
DOI: 10.1016/j.fitote.2025.106376

Abstract

Four previously undescribed diterpenoids (2-5) were isolated from the bark of Torreya grandis, along with ten known analogues. The structures of the compounds were elucidated using NMR, HR-ESIMS, and ECD calculation. The cytotoxic effects of the isolated compounds on HCT-116, AsPC-1, and HepG2 cells were evaluated using the MTT assay. Compound 6 exhibited significant cytotoxicity against HepG2 cells. Further experiments revealed that compound 6 could arrest the G2 phase process in HepG2 cells through the JAK2/STAT3 signaling pathway.

Keywords: Cytotoxicity; Diterpenoid; HepG2; Taxaceae; Torreya grandis.