Regulation of important natural products biosynthesis by WRKY transcription factors in plants

Background: Plants produce abundant natural products, among which are species-specific and diversified secondary metabolites that are essential for growth and development, as well as adaptation to adversity and ecology. Moreover, these secondary metabolites are extensively utilized in pharmaceuticals, fragrances, industrial materials, and more. WRKY transcription factors (TFs), as a family of TFs unique to plants, have significant functions in many plant life activities. Especially in recent years, their role in the field of secondary metabolite biosynthesis regulation has received much attention. However, very little comprehensive summarization has been done to review their research progress.

Aim of review: The purpose of this work is not only to provide valuable insights into the regulation of WRKY TFs over metabolic pathways through compiling the WRKY TFs involved in these processes, but also to offer research directions for WRKY TFs by summarizing the regulatory modes of WRKY TFs in the biosynthesis of secondary metabolites, thereby increasing the yield of valuable natural products in the future.

Key scientific concepts of review: Secondary metabolites can be categorized into three major classes-terpenoids, phenolic compounds, and nitrogen-containing compounds-based on their structural characteristics and biosynthetic pathways, and further subdivided into numerous subclasses. We review in detail the research progressregardingthe regulatory roles of WRKY TFs in plant secondary metabolitebiosynthesis and summarize more than 40 major related species. Additionally, we have presented the concepts of action modes of WRKY TFs involved in metabolic pathways, including direct regulation, indirect regulation, co-regulation, and self-regulation. It is helpful for others to investigate the molecular mechanisms of TF-mediated regulation. Furthermore, regarding future research prospects, we believe that research in this area lays the foundation for increasing the yield of important plant-derived natural products by molecular breeding, generating significant economic and social benefits.