Evaluation of QuantiFERON-TB Gold for the Diagnosis of Mycobacterium tuberculosis Infection in HTLV-1-Infected Patients

Viruses. 2024 Nov 30;16(12):1873. doi: 10.3390/v16121873.

Abstract

Human T-cell leukemia virus type 1 (HTLV-1) is associated with an increased risk of tuberculosis (TB). This study aimed to evaluate the performance of the QuantiFERON-TB Gold (QFT) test for the diagnosis of Mycobacterium tuberculosis (MTB) infection in HTLV-1-infected individuals. HTLV-1-infected participants were divided into four groups: HTLV-1-infected individuals with a history of tuberculosis (HTLV/TB), individuals with positive HTLV and tuberculin skin tests (HTLV/TST+) or negative TST (HTLV/TST-), and HTLV-1-negative individuals with positive TST results (HN/TST+). We compared the diagnostic performance of the QFT assay with that of the TST as a reference and evaluated test sensitivity, specificity, accuracy, likelihood ratio, and diagnostic odds ratio. The results showed a higher frequency of positive TST results and induration diameter ≥10 mm in HTLV-1-infected individuals than in the controls. The QFT test was more frequently positive in the HTLV/TB group than in the other groups, while a combined analysis of HTLV/TB and HTLV/TST+ indicated a QFT sensitivity of 57.5%. No significant differences were found in the other diagnostic performance measures, as QFT test results were in agreement with TST results, particularly in TST-negative individuals. Given the low sensitivity of QFT for LTBI in individuals infected with HTLV-1, the TST may be preferable in regions where both infections are endemic.

Keywords: HTLV-1; QuantiFERON; tuberculin skin test; tuberculosis.

Publication types

  • Evaluation Study

MeSH terms

  • Adult
  • Aged
  • Coinfection / diagnosis
  • Coinfection / microbiology
  • Coinfection / virology
  • Female
  • HTLV-I Infections* / complications
  • HTLV-I Infections* / diagnosis
  • Human T-lymphotropic virus 1* / immunology
  • Human T-lymphotropic virus 1* / isolation & purification
  • Humans
  • Interferon-gamma Release Tests* / methods
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis* / immunology
  • Sensitivity and Specificity*
  • Tuberculin Test / methods
  • Tuberculosis* / diagnosis