Does a priming warm-up influence the incidence of [Formula: see text] during a ramp test and verification phase?

PLoS One. 2025 Jan 8;20(1):e0313698. doi: 10.1371/journal.pone.0313698. eCollection 2025.

Abstract

Objective: This study compared the effects of two different warm-up protocols (normal vs. priming) on the oxygen plateau ([Formula: see text]) incidence rate during a ramp test. It also compared the cardiopulmonary responses during the ramp test and subsequent verification phase.

Methods: Eleven recreational cyclists performed two experimental visits. The first visit required a normal warm-up (cycling at 50 W for 10 min) followed by the ramp test (30 W.min-1) and supramaximal verification phase with 30 min rest between tests. The second visit required a priming warm-up (cycling at 50 W for 4 min increasing to 70% difference between the gas exchange threshold [GET] and maximum work rate [WRmax] for 6 min) followed by the same protocol as in the first visit. Physiological responses were collected during the exercise and compared. Oxygen kinetics ([Formula: see text] Kinetics) and [Formula: see text] incidence rate were determined during the ramp tests for both visits.

Results: As planned, following the warm-up the priming visit experienced greater physiological response. However, the incidence rate of [Formula: see text] during the ramp test was the same between visits (73%), and maximal oxygen uptake was not different between visits after the ramp test (normal, 4.0 ± 0.8; primed, 4.0 ± 0.7 L·min-1, p = 0.230) and verification phase (normal, 3.8 ± 0.6; primed, 3.8 ± 0.7 L·min-1, p = 0.924) using a Holm-Bonferroni correction for controlling family-wise error rate. [Formula: see text] Kinetics were not different between visits during the ramp test (normal, 10.8 ± 1.1; primed, 10.8 ± 1.2 mL·min-1·W-1, p = 0.407). The verification phase confirmed [Formula: see text] in 100% for both the normal and priming visits.

Conclusion: Our hypothesis that a priming warm-up facilitates the incidence rate of [Formula: see text] during a ramp test is not supported by the results. The verification phase remains a prudent option when determining a 'true' [Formula: see text] is required.

MeSH terms

  • Adult
  • Bicycling / physiology
  • Exercise Test / methods
  • Humans
  • Male
  • Oxygen / metabolism
  • Oxygen Consumption*
  • Warm-Up Exercise*
  • Young Adult

Substances

  • Oxygen

Grants and funding

This research was supported by the Mark Diamond Research Fund, University at Buffalo (SP-23-08) to JianBo Qiao (https://ubwp.buffalo.edu/gsa/mdrf/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.