Comparing the antioxidant effects of single and binary combinations of Lactiplantibacillus plantarum in vitro and in vivo and their application in yogurt

Kun Yang; Bingjing Zhou; Qianping Xu; Yingtong Li; Junqing Lin; Yang Zhou; Mingqi Liu; Jin Zhao; Yang Zhu; Xianjun Dai

doi:10.3168/jds.2024-25722

Comparing the antioxidant effects of single and binary combinations of Lactiplantibacillus plantarum in vitro and in vivo and their application in yogurt

J Dairy Sci. 2025 Jan 6:S0022-0302(24)01443-7. doi: 10.3168/jds.2024-25722. Online ahead of print.

Authors

Kun Yang¹, Bingjing Zhou¹, Qianping Xu¹, Yingtong Li¹, Junqing Lin¹, Yang Zhou¹, Mingqi Liu¹, Jin Zhao¹, Yang Zhu², Xianjun Dai³

Affiliations

¹ Key Laboratory of Specialty Agri-product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, 310018, China.
² School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, 310018, China. Electronic address: [email protected].
³ Key Laboratory of Specialty Agri-product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, 310018, China. Electronic address: [email protected].

PMID: 39778801
DOI: 10.3168/jds.2024-25722

Abstract

Lactiplantibacillus plantarum (L. plantarum) have been studied for their antioxidant properties, which can mitigate oxidative stress and improve health outcomes. The study aimed to compare the antioxidant properties of single and binary L. plantarum and their impact on yogurt. L. plantarum 847 (Lp-C), L. plantarum 8014 (Lp-G), and their combination were chosen for their in vitro antioxidant potential. In vivo experiments were performed in Drosophila melanogaster (D. melanogaster) and results showed that binary L. plantarum significantly improved the survival time, weight, catalase activity and intestinal integrity in H₂O₂-induced flies. As compared with single L. plantarum treated flies, binary strains improved the survival curve, superoxide dismutase and catalase activities in females, prolonged the average survival time in males, and increased the expression level of keap1, Nrf2 and SOD genes in all genders. To explore the effect of single and binary L. plantarum on milk fermentation, the physicochemical properties and antioxidant activity of yogurt were detected, and results presented that yogurt fermented with L. plantarum exhibited the improved antioxidant capacity, with the binary strain combination demonstrating superior effects in rheological properties and the later period of yogurt storage. This research offers a foundation for choosing the combinations of lactic acid bacteria (LAB) with antioxidant properties.

Keywords: Antioxidant activity; Lactiplantibacillus plantarum; Yogurt.

© 2025, The Authors. Published by Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).