Effect of ultrasound intervention timing on the formation mechanism and emulsifying properties of chickpea protein isolate-citrus pectin complexes

This study systematically investigated the effects of ultrasonic treatment timing on the formation and emulsifying properties of chickpea protein isolate (CPI) and citrus pectin (CP) complexes. Results showed that introducing ultrasound during the CPI pre-treatment stage significantly enhanced the emulsifying activity and stability of the complexes. Notably, compared with the independent CPI, the emulsifying activity index of CPIU-CP soared from 0.14 m²/g to 0.26 m²/g, while the emulsion stability index increased dramatically from 41.5 % to 95.5 %. Mechanistic studies revealed that ultrasonic treatment disrupted the protein structure of CPI, increasing its surface hydrophobicity and charge, thereby enhancing electrostatic interactions and steric hindrance effects with CP. Consequently, the interfacial properties of the complex were improved, leading to enhanced emulsifying performance. This study provides a novel approach to utilize ultrasound technology to regulate the structure and function of protein-polysaccharide complexes.