The sensor protein VdSLN1 is involved in regulating melanin biosynthesis and pathogenicity via MAPK pathway in Verticillium dahliae

The vascular wilt fungus Verticillium dahliae is a destructive soil-borne pathogen that causes yield loss on various economically important crops. Membrane-spanning sensor protein SLN1 have been demonstrated to contribute to virulence in varying degrees among numerous devastating fungal pathogens. However, the biological function of SLN1 in V. dahliae remains unclear. In this study, we identified the membrane-spanning sensor protein encoding gene VdSLN1 and it interacts physically with Vst50 and regulates the expression of MAPK module Vst50-Vst11-Vst7. The expression of VdSLN1 was also positively regulated by the MAPK signaling pathways transmembrane-associated members VdSho1 and VdMsb2, suggesting that the expression of VdSLN1 is associated with VdSho1 and VdMsb2. In addition, we found that VdSLN1, similar to VdSho1 and VdMsb2, is not required for V. dahliae vegetative growth and response to various abiotic stresses. While, ΔVdSLN1 mutant exhibited slightly reduced ability to penetrate a cellophane membrane and melanin synthesis compared with the wild type strain. Further experiments indicate that VdSLN1, VdSho1 and VdMsb2 has an additive effect on the virulence, cellophane penetration and melanin biosynthesis and of V. dahliae. In short, VdSLN1, though not essential, plays a role in cellophane penetration, melanin biosynthesis, also contributes to the virulence, as the downstream factor of VdSho1 and VdMsb2.