Phenotypic analysis of complex bioengineered 3D models

Akhilandeshwari Ravichandran; Vaibhav Mahajan; Tom van de Kemp; Anna Taubenberger; Laura J Bray

doi:10.1016/j.tcb.2024.12.004

Phenotypic analysis of complex bioengineered 3D models

Trends Cell Biol. 2025 Jan 9:S0962-8924(24)00257-5. doi: 10.1016/j.tcb.2024.12.004. Online ahead of print.

Authors

Akhilandeshwari Ravichandran¹, Vaibhav Mahajan², Tom van de Kemp¹, Anna Taubenberger², Laura J Bray³

Affiliations

¹ Centre for Biomedical Technologies, Queensland University of Technology (QUT), Brisbane, QLD 4059, Australia; School of Mechanical, Medical, and Process Engineering, Queensland University of Technology (QUT), Brisbane, QLD 4059, Australia.
² Biotechnology Center, Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden, 01307 Dresden, Germany.
³ Centre for Biomedical Technologies, Queensland University of Technology (QUT), Brisbane, QLD 4059, Australia; School of Mechanical, Medical, and Process Engineering, Queensland University of Technology (QUT), Brisbane, QLD 4059, Australia; Australian Research Council (ARC) Training Centre for Cell and Tissue Engineering Technologies, Queensland University of Technology (QUT), Brisbane, QLD 4059, Australia. Electronic address: [email protected].

PMID: 39794253
DOI: 10.1016/j.tcb.2024.12.004

Abstract

With advances in underlying technologies such as complex multicellular systems, synthetic materials, and bioengineering techniques, we can now generate in vitro miniaturized human tissues that recapitulate the organotypic features of normal or diseased tissues. Importantly, these 3D culture models have increasingly provided experimental access to diverse and complex tissues architectures and their morphogenic assembly in vitro. This review presents an analytical toolbox for biological researchers using 3D modeling technologies through which they can find a collation of currently available methods to phenotypically assess their 3D models in their normal state as well as their response to therapeutic or pathological agents.

Keywords: 3D tissue models; bioengineered tissues; gene and protein profiling; mechanical assessment; microscopy; phenotypic analysis.

Publication types

Review