Innovative submerged directed fermentation: Producing high molecular weight polysaccharides from Ganoderma lucidum

Jia Guo; Chenmin Tang; Yanfang Liu; Jia Shi; Jovana Vunduk; Chuanhong Tang; Jie Feng; Jingsong Zhang

doi:10.1016/j.foodchem.2025.142759

Innovative submerged directed fermentation: Producing high molecular weight polysaccharides from Ganoderma lucidum

Food Chem. 2025 Jan 4:471:142759. doi: 10.1016/j.foodchem.2025.142759. Online ahead of print.

Authors

Jia Guo¹, Chenmin Tang², Yanfang Liu², Jia Shi², Jovana Vunduk³, Chuanhong Tang², Jie Feng⁴, Jingsong Zhang⁵

Affiliations

¹ Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, China. National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Fengxian District, Shanghai 201403, China; School of Health Science and Engineering, Shanghai Engineering Research Center of Food Microbiology, University of Shanghai for Science and Technology, Shanghai 200093, China.
² Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, China. National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Fengxian District, Shanghai 201403, China.
³ Institute of General and Physical Chemistry, Studenski trg 10-12, 11 158 Belgrade, Serbia.
⁴ Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, China. National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Fengxian District, Shanghai 201403, China. Electronic address: [email protected].
⁵ Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, China. National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Fengxian District, Shanghai 201403, China. Electronic address: [email protected].

PMID: 39799682
DOI: 10.1016/j.foodchem.2025.142759

Abstract

Polysaccharides from Ganoderma lucidum (GLPs) exhibit unique bioactivity, but traditional cultivation yields low quantities and unstable quality, limiting their research and application. This study highlights how submerged fermentation processes enable the directed acquisition of structurally defined high molecular weight (MW) bioactive intracellular polysaccharides (IPS). The results showed that inoculation amount and fermentation scales had a significant effect on the content of high MW IPS. In the fermentor, by lowering the initial glucose concentration combined with fed-batch fermentation, the high MW IPS content was improved. The monosaccharide composition indicated that the high MW IPS obtained from different fermentation scales exhibited stability. This polysaccharide, which is a β-glucan with a β-1,3-Glcp backbone and β-1-Glcp attached at the O-6 position, demonstrated immunostimulatory effects in vitro. Overall, the consistent quality of GLPs during submerged fermentation underscores the feasibility of industrial-scale production, presenting a significant advancement over traditional cultivation methods and promising for biotechnological applications.

Keywords: Ganoderma lucidum; High molecular weight polysaccharide; Submerged fermentation.