Development of fluorescent-photothermal probe based on photoinduced energy transfer: A dual-readout immunosensor for the detection of illegal additive

Hongzhi Liang; Aori Qileng; Haoran Shen; Ziyi Zhang; Weipeng Liu; Zhen-Lin Xu; Yingju Liu

doi:10.1016/j.bios.2025.117140

Development of fluorescent-photothermal probe based on photoinduced energy transfer: A dual-readout immunosensor for the detection of illegal additive

Biosens Bioelectron. 2025 Jan 7:273:117140. doi: 10.1016/j.bios.2025.117140. Online ahead of print.

Authors

Hongzhi Liang¹, Aori Qileng¹, Haoran Shen¹, Ziyi Zhang², Weipeng Liu³, Zhen-Lin Xu⁴, Yingju Liu⁵

Affiliations

¹ Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China; Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
² Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.
³ Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China. Electronic address: [email protected].
⁴ Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
⁵ Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China; Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China. Electronic address: [email protected].

PMID: 39808993
DOI: 10.1016/j.bios.2025.117140

Abstract

The development of advanced optical probes for point-of-care testing holds great importance in the field of diagnostic technologies. This study focused on the synthesis of a probe featuring both fluorescent and photothermal responses with single excitation wavelength, which was achieved through the combination of oxidized camellia oleifera shell powder (OC) and Prussian blue nanoparticles (PBNPs). Notably, OC derived from the direct processing of raw material showed fluorescent and phosphorescent emissions simultaneously, and the positions of the two peaks overlapped with the absorbance range of PBNPs, making the fluorescent and phosphorescent emissions of OC effectively quenched by PBNPs. Meanwhile, the photothermal property of PBNPs was activated by the phosphorescent emission of OC through photoinduced energy transfer. As a proof of concept, OC@PBNPs was applied in the dual-channel immunoassay, in which illegal addictive aminopyrine (AP) was chosen as the detection target. Furthermore, a portable device was developed to capture the fluorescent and photothermal signals of OC@PBNPs, rendering the detection method based on OC@PBNPs suitable for point-of-care testing (POCT).

Keywords: Immunosensor; Oxidized lignin; Photothermal; Point-of-care testing; Prussian blue.