Chloroplast alchemy: rewriting the chloroplast genome with high precision

Trends Plant Sci. 2025 Jan 17:S1360-1385(24)00350-9. doi: 10.1016/j.tplants.2024.12.014. Online ahead of print.

Abstract

In the past decade, precise genome editing has transitioned from theory to practical application. Yet, plastid genomes have largely remained impervious to these advances. A recent breakthrough by Mok et al. (2024) demonstrated successful plastid genome editing by developing transgene-free atrazine-resistant arabidopsis (Arabidopsis thaliana) plants.

Keywords: chloroplast genome editing; high-precision; precise editing; transgene-free.