Real-time monitoring of ONOO⁻ in cerebral ischemia-reperfusion injury mouse models using a hydrazine-based NIR fluorescent probe

Jinting Shang; Yan Yang; Yaojian Sun; Wanxia Gao; Kang Ma; Chen Wang; Xin Yu; Liping Li; Jiang Zheng; Na Zhao; XiJi Shu; Yibin Zhang

doi:10.1016/j.redox.2025.103494

Real-time monitoring of ONOO⁻ in cerebral ischemia-reperfusion injury mouse models using a hydrazine-based NIR fluorescent probe

Redox Biol. 2025 Jan 13:80:103494. doi: 10.1016/j.redox.2025.103494. Online ahead of print.

Authors

Jinting Shang¹, Yan Yang², Yaojian Sun², Wanxia Gao², Kang Ma², Chen Wang², Xin Yu³, Liping Li², Jiang Zheng², Na Zhao², XiJi Shu⁴, Yibin Zhang⁵

Affiliations

¹ Hubei Key Laboratory of Cognitive and Affective Disorders, Institute of Biomedical Sciences, School of Medicine, Jianghan University, Wuhan, 430056, China. Electronic address: [email protected].
² Hubei Key Laboratory of Cognitive and Affective Disorders, Institute of Biomedical Sciences, School of Medicine, Jianghan University, Wuhan, 430056, China.
³ Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
⁴ Hubei Key Laboratory of Cognitive and Affective Disorders, Institute of Biomedical Sciences, School of Medicine, Jianghan University, Wuhan, 430056, China. Electronic address: [email protected].
⁵ Hubei Key Laboratory of Cognitive and Affective Disorders, Institute of Biomedical Sciences, School of Medicine, Jianghan University, Wuhan, 430056, China; College of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing, China. Electronic address: [email protected].

PMID: 39827589
DOI: 10.1016/j.redox.2025.103494

Abstract

Accurate and selective techniques for visualizing endogenous peroxynitrite (ONOO^-) in cerebral ischemia-reperfusion injury (CIRI) models are essential for understanding its complex pathological processes. Here, we introduced a longwave fluorescent probe TJO for detecting ONOO^- rapidly and sensitively, with a low detection limit of 91 nM. Furthermore, TJO exhibits excellent fluorescence imaging capabilities, enabling detailed visualization of ONOO⁻ in CIRI mice model. This highlights its potential for real-time monitoring of ONOO⁻-related pathological conditions.

Keywords: Cerebral ischemia-reperfusion injury (CIRI); Fluorescent probe; Imaging; Peroxynitrite.