Stress-responsive plasma membrane H+-ATPases regulate deep rooting in rice

Di Wang; Kai Xu; Shoujun Chen; Lei Wang; Qiaojun Lou; Changsen Zhong; Yawen Wang; Tiemei Li; Huaxiang Cheng; Lijun Luo; Liang Chen

doi:10.1016/j.plantsci.2025.112394

Stress-responsive plasma membrane H⁺-ATPases regulate deep rooting in rice

Plant Sci. 2025 Jan 17:112394. doi: 10.1016/j.plantsci.2025.112394. Online ahead of print.

Authors

Di Wang¹, Kai Xu², Shoujun Chen³, Lei Wang², Qiaojun Lou¹, Changsen Zhong¹, Yawen Wang¹, Tiemei Li⁴, Huaxiang Cheng⁴, Lijun Luo⁵, Liang Chen⁶

Affiliations

¹ College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China; Shanghai Agrobiological Gene Center, Shanghai, 201106, China.
² Shanghai Agrobiological Gene Center, Shanghai, 201106, China; Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, China.
³ College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China; Shanghai Agrobiological Gene Center, Shanghai, 201106, China; Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, China.
⁴ Shanghai Agrobiological Gene Center, Shanghai, 201106, China.
⁵ College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China; Shanghai Agrobiological Gene Center, Shanghai, 201106, China; Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, China. Electronic address: [email protected].
⁶ College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China; Shanghai Agrobiological Gene Center, Shanghai, 201106, China; Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, China. Electronic address: [email protected].

PMID: 39827950
DOI: 10.1016/j.plantsci.2025.112394

Abstract

Agricultural production is severely affected by environmental stresses such as drought, and deep rooting is an important factor enhancing crop drought avoidance. H⁺-ATPases provide a transmembrane proton gradient and are thought to play a crucial role in plant growth and abiotic stress responses. However, their expression under abiotic stress and function on deep rooting is poorly understood in rice. In this study, the conserved domains, potential phosphorylation sites, and three-dimensional structures of ten Oryza sativa PM H⁺-ATPases (OSAs) were analyzed. Quantitative PCR analysis revealed different expression patterns of these OSA genes under hormone treatment conditions (e.g., abscisic acid) and abiotic stress conditions (e.g., drought and salt stress). Subcellular localization analysis revealed that most OSA proteins were localized to the cell membrane. Phenotype determination of OSA mutants indicated that the ratio of deep rooting (RDR) of both osa7 and osa8 mutants was significantly reduced compared to that of wild-type rice plants. Additionally, OSA haplotypes in 268 rice accessions were analyzed, and the haplotypes associated with RDR were identified. The present results provide valuable information on crucial domains, expression patterns, and functional identification of OSA paralogs to reveal their role in rice responses to abiotic stress.

Keywords: H(+)-ATPases; abiotic stresses; deep rooting; drought stress; expression; rice.