Isolation and Evaluation of Potential Use of Prebiotics-Utilizing Butyrate-Producing Bacteria in Nibea coibor

Zhongzhen Li; Ngoc Tuan Tran; Ming Zhang; Zhaoxi Li; Wanying Yang; Shuqi Wang; Zhong Hu; Shengkang Li

doi:10.1155/anu/4679037

Isolation and Evaluation of Potential Use of Prebiotics-Utilizing Butyrate-Producing Bacteria in Nibea coibor

Aquac Nutr. 2025 Jan 11:2025:4679037. doi: 10.1155/anu/4679037. eCollection 2025.

Authors

Zhongzhen Li^{1

2

3

4

5}, Ngoc Tuan Tran^{1

2

3}, Ming Zhang^{1

2

3}, Zhaoxi Li^{1

2}, Wanying Yang⁴, Shuqi Wang^{1

2

3}, Zhong Hu^{1

2

3}, Shengkang Li^{1

2

3}

Affiliations

¹ Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China.
² Institute of Marine Sciences, Shantou University, Shantou 515063, China.
³ STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China.
⁴ Zhuhai Institute of Translational Medicine, Zhuhai People's Hospital (Zhuhai Clinical Medical College of Jinan University), Jinan University, Zhuhai 519000, China.
⁵ Guangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China.

Abstract

Butyrate-producing bacteria (BPB) benefit the health of aquatic animals. This current study aimed to isolate BPB from the intestines of Nibea coibor and assess their probiotic potential. The results showed that nine isolates were obtained in vitro from the gut of N. coibor, including six Clostridium butyricum, two Proteocatella sphenisci, and one Fusobacterium varium. The representative bacteria, C. butyricum CG-3 and P. sphenisci DG-1, which produce high butyrate levels, were further studied for short-chain fatty acid (SCFA) production and antibiotic susceptibility. The effects of BPB singly (CB: basal diet + CG-3 and PS: basal diet + DG-1, at 10⁷ CFU/g) or in combination with galactooligosaccharides (GOS) (0.5%) and inulin (0.5%) (CBIG) or D-sorbitol (0.5%) (PSGS) on the growth and health status of N. coibor were investigated. Results showed an increase in growth parameters in the CB, CBIG, and PSGS groups, except for the PS group. Alterations in intestinal microbiota (including diversity, abundance, and function) were observed in four experimental groups (CB, CBIG, PS, and PSGS groups). SCFA contents increased in treated groups; butyrate production was positively related to bacterial abundance. Compared to control, levels of complement C3, complement C4, immunoglobulin M (IgM), transforming growth factor-β (TGF-β), interleukin (IL)-10, IL-1β, and lysozyme (LZM) increased, while malondialdehyde (MDA) decreased in treated groups. Contents of IL-6 (PS and PSGS groups), tumor necrosis factor-alpha (TNF-α) (CB, PS, and PSGS groups), total antioxidant capacity (T-AOC) (CB and PS groups), total superoxide dismutase (T-SOD) (PS group), catalase (CAT) (CB and PSGS groups), and activities of amylase (PS and PSGS groups), trypsin (CB group), and lipase (CBIG group) were increased. Our results suggested the potential use of C. butyricum CG-1 or P. sphenisci DG-1 singly or in combination with prebiotics improved growth and health conditions in N. coibor.

Keywords: Nibea coibor; butyrate-producing bacteria; growth; gut microbiota; immune response; short-chain fatty acids.