Effects of photobiomodulation therapy (660-nm laser) on median nerve function in Wistar rats

This study investigates the effects of photobiomodulation (PBM) with a 660-nm laser on nerve regeneration and muscle morphometry following median nerve axonotmesis in rats. Sixteen Wistar rats were divided into a control group and laser-treated group, with the latter receiving 10 applications of PBM (660 nm; 20 mW; 10 J/cm²; 0.4 J; and 20 s) over 2 weeks. Functional analysis was performed using the grasping test, which measures the grip strength of the forelimb digits to evaluate motor function of the median nerve. Morphometric analyses were conducted on the median nerve, flexor digitorum (FD) muscle, and flexor carpi radialis (FCR) muscle. PBM led to functional improvement, with significant differences observed on the 21st day post-injury. However, no significant differences between groups were found in nerve morphometry. Morphometric analysis of the FD muscle revealed significant improvements in the PBM group, but with no significant differences regarding the FCR muscle. These findings suggest that 660-nm laser with the parameters used produced functional improvement as well as significant improvements in the morphometry of the FD muscle, but did not have significant effects on the morphometry of the FCR muscle or nerve regeneration.