Chromosome-scale genomes of ecologically and economically important rabbitfish Siganus guttatus and Siganus oramin

Lin Xian; Sunil Kumar Sahu; Xiaolin Huang; Chao Li; Yu Liang; Yan Ou-Yang; Huayang Guo; Bo Liu; Kecheng Zhu; Baosuo Liu; Nan Zhang; Tengfei Zhu; Qiye Li; Dianchang Zhang

doi:10.1016/j.ygeno.2025.111007

Chromosome-scale genomes of ecologically and economically important rabbitfish Siganus guttatus and Siganus oramin

Genomics. 2025 Jan 23:111007. doi: 10.1016/j.ygeno.2025.111007. Online ahead of print.

Authors

Lin Xian¹, Sunil Kumar Sahu², Xiaolin Huang³, Chao Li⁴, Yu Liang³, Yan Ou-Yang³, Huayang Guo³, Bo Liu³, Kecheng Zhu³, Baosuo Liu³, Nan Zhang³, Tengfei Zhu³, Qiye Li⁵, Dianchang Zhang⁶

Affiliations

¹ Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; Sanya Tropical Fisheries Research Institute, Sanya 572018, China; State Key Laboratory of Agricultural Genomics, BGI-Shenzhen, Shenzhen 518083, China.
² State Key Laboratory of Agricultural Genomics, BGI-Shenzhen, Shenzhen 518083, China.
³ Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; Sanya Tropical Fisheries Research Institute, Sanya 572018, China; Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, China.
⁴ Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Engineering Technology Research Center for Environmentally Friendly Aquaculture, School of Life Sciences, South China Normal University, Guangzhou, China.
⁵ State Key Laboratory of Agricultural Genomics, BGI-Shenzhen, Shenzhen 518083, China. Electronic address: [email protected].
⁶ Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; Sanya Tropical Fisheries Research Institute, Sanya 572018, China; Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, China. Electronic address: [email protected].

PMID: 39863185
DOI: 10.1016/j.ygeno.2025.111007

Abstract

Siganus guttatus and Siganus oramin are two major species that are naturally distributed along the Eastern Pacific coast and possess considerable ecological and economic value. Here, we present the construction and comparative analysis of the chromosome-level genomes of these two Siganus species. Employing a hybrid assembly strategy, we partitioned and independently assembled the PacBio, Illumina and Hi-C reads of S. guttatus and S. oramin, resulting in chromosome-level genomes. The assembly sizes (N50 size, BUSCO completeness) of the two genomes were 642.4 M (25.76 M, 98.26 %) and 502.8 M (16.98 M, 98.8 %) for S. guttatus and S. oramin, respectively, exhibiting high contiguity and integrity. This study marks the first successful assembly of chromosome-level genomes in Siganus species, along with an initial exploration of their dietary habits and habitats through comparative genomics analysis. These findings offer essential resources for comparative genomics and molecular evolution research.

Keywords: Fish; Genome; HiC; Siganus guttatus; Siganus oramin.