Abstract
The translational inhibition produced by addition of oxidized glutathione (GSSG) to hemin-containing reticulocyte lysates and the accompanying phosphorylation of the alpha subunit of the polypeptide chain initiation factor eIF-2 can be prevented or reversed by NADPH generators, including glucose 6-phosphate, deoxyglucose 6-phosphate, fructose 6-phosphate, NADPH itself, and also by dithiols, e.g., dithiothreitol, but not by reduced glutathione (GSH) or other monothiols, e.g., 2-mercaptoethanol. The same is true of the inhibition caused by addition of glutamate dehydrogenase, alpha-ketoglutarate, and NH4+, which may be entirely due to NADPH depletion via the reaction.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Ammonia / pharmacology
-
Animals
-
Dithiothreitol / pharmacology
-
Electrophoresis, Polyacrylamide Gel
-
Enzyme Activation
-
Eukaryotic Initiation Factor-2
-
Glucose-6-Phosphate
-
Glucosephosphates / pharmacology
-
Glutamate Dehydrogenase / metabolism
-
Glutathione / analogs & derivatives*
-
Glutathione / pharmacology
-
Glutathione Disulfide
-
Heme / pharmacology*
-
Ketoglutaric Acids / pharmacology
-
NADP / metabolism*
-
Peptide Initiation Factors / metabolism
-
Protein Biosynthesis / drug effects
-
Protein Kinases / metabolism*
-
Proteins / metabolism
-
Rabbits
-
Reticulocytes / enzymology
-
eIF-2 Kinase
Substances
-
Eukaryotic Initiation Factor-2
-
Glucosephosphates
-
Ketoglutaric Acids
-
Peptide Initiation Factors
-
Proteins
-
Heme
-
NADP
-
Glucose-6-Phosphate
-
Ammonia
-
Glutamate Dehydrogenase
-
Protein Kinases
-
eIF-2 Kinase
-
Glutathione
-
Dithiothreitol
-
Glutathione Disulfide