Bromodeoxyuridine treatment of rat liver cells in culture induces an increased metabolic conversion of exogenous ornithine

Biochem Biophys Res Commun. 1985 Jun 28;129(3):746-52. doi: 10.1016/0006-291x(85)91955-2.

Abstract

In the presence of bromodeoxyuridine, rat liver cells show an increased incorporation of radiolabeled ornithine into cellular proteins. This increase was not due to activation of urea cycle enzymes but rather to an increase in metabolism of ornithine. Furthermore, treatment with bromodeoxyuridine changes profoundly and selectively the size of the free amino acid pool in these cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Autoradiography
  • Bromodeoxyuridine / pharmacology*
  • Cell Division / drug effects
  • Cells, Cultured
  • Cycloheximide / pharmacology
  • Epithelium / metabolism
  • Fibroblasts / metabolism
  • Liver / drug effects*
  • Liver / metabolism
  • Ornithine / metabolism*
  • Rats

Substances

  • Amino Acids
  • Cycloheximide
  • Ornithine
  • Bromodeoxyuridine