The effects of 2-deoxyglucose (2-DOG), alpha-methylglucoside (alpha-MG), and glucosamine (GA) on aflatoxin production by Aspergillus parasiticus were studied using conidia-initiated and replacement cultures. In conidia-initiated, 2-DOG, alpha-MG, and GA supported varying amounts of growth when employed as sole carbon sources. In both conidia-initiated and replacement cultures, 2-DOG, but not alpha-MG nor GA, as sole carbon sources support toxin formation. None of the compounds inhibited aflatoxin production when used in combination with glucose. It appears that neither 2-DOG, alpha-MG, nor GA can be considered nonmetabolizable analogs of glucose in A. parasiticus.