Regulation of bacteriophage mu and mini-mu DNA replication in vivo

Biochem Int. 1985 Jun;10(6):945-53.

Abstract

To study the regulation of bacteriophage Mu DNA's integrative-replication (transposition) during lytic growth in a cell containing both a Mu and a helper-dependent Mini-Mu (short, internally-deleted Mu genome), we placed "marker" genes (bla, lacZ) within either genome and then measured their encoded enzymes as indicators of the gene dosage. These results, corroborated using DNA-DNA hybridization, show that Mu and Mini-Mu DNA transposition is well regulated, requires both the Mu A and B gene products, and can be readily monitored by measuring beta-galactosidase and beta-lactamase expressed from the lacZ and bla genes, respectively.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Coliphages / genetics*
  • DNA Replication*
  • DNA, Viral / genetics*
  • Genotype
  • Nucleic Acid Hybridization
  • Virus Replication*

Substances

  • DNA, Viral