Abstract
An antineoplastic factor inducing tumor lysis was purified to apparent homogeneity from the supernatant of a homogenate of eggs of the sea hare Aplysia kurodai. On gel filtration, the purified substance gave a single band of material of 250 kDa containing 3 different subunits and this band coincided with cytolytic activity. The purified factor was half-maximally active at 10-60 ng protein/ml and lysed all tumor cells tested, but not normal cells.
MeSH terms
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Animals
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Antineoplastic Agents / isolation & purification*
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Antineoplastic Agents / pharmacology
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Aplysia / analysis*
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Carbohydrates / analysis
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Cell Line
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Chemical Phenomena
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Chemistry
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Chromatography, Gel
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Electrophoresis, Polyacrylamide Gel
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Female
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Humans
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Leukemia L1210 / drug therapy
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Male
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Mice
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Mice, Inbred Strains
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Neoplasms, Experimental / drug therapy
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Ovum / analysis
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Proteins / analysis
Substances
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Antineoplastic Agents
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Carbohydrates
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Proteins