Use of argA-lac fusions to generate lambda argA-lac bacteriophages and to determine the direction of argA transcription in Escherichia coli

J Bacteriol. 1977 Oct;132(1):60-6. doi: 10.1128/jb.132.1.60-66.1977.

Abstract

Fusions of lac genes to the argA operator were constructed, and lambda phages carrying these fusions were isolated and characterized. With the aid of a lambda phage carrying an argA-lac fusion, the direction of argA transcription on the Escherichia coli chromosome was determined to be clockwise.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arginine / biosynthesis
  • Arginine / genetics*
  • Chromosomes, Bacterial*
  • Coliphages / genetics*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Genes, Viral
  • Lactose / metabolism
  • Lysogeny
  • Operon
  • Transcription, Genetic*
  • Transduction, Genetic
  • beta-Galactosidase / biosynthesis

Substances

  • Arginine
  • beta-Galactosidase
  • Lactose