Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport

Biochem J. 1979 Apr 15;180(1):37-44. doi: 10.1042/bj1800037.

Abstract

A new method for studying membrane transport is presented. High resolution n.m.r. is used to measure the distribution of small molecules between the intracellular and extracellular compartments. The method uses spin-echo techniques and relies on a difference in the magnetic susceptibility of the media inside and outside of cells. It also provides simultaneous information on the metabolic status of the cell. The method is illustrated by a study of alanine and lactate transport in the human erythrocyte.

MeSH terms

  • Alanine / blood
  • Biological Transport
  • Cell Membrane / metabolism*
  • Erythrocytes / metabolism
  • Humans
  • Lactates / blood
  • Magnetic Resonance Spectroscopy

Substances

  • Lactates
  • Alanine