The effects of chemical form of supplemental zinc and elevated dietary calcium on intestinal absorption of zinc were measured. Calves and rats were fed diets low in zinc, and zinc availability was the percentage increase of zinc in plasma with dietary supplementation of zinc. Availabilities of zinc supplied as zinc chloride, zinc sulfate, zinc oxide, and zinc carbonate were comparable in both calves and rats. Elevated amounts of dietary calcium as ground limestone reduced absorption of zinc in rats fed soy-protein but had no effect on absorption of zinc in the lactating cow. Thus, for cows consuming large amounts of calcium, an increase in the concentration of zinc beyond 40 ppm of the diet appears unnecessary.