Expression and segregation of HL-A antigens in D98/AH-2 by lymphocyte and fibroblast hybrids

Tissue Antigens. 1975 Aug;6(2):80-92. doi: 10.1111/j.1399-0039.1975.tb00619.x.

Abstract

Somatic cell hybrids between D98/AH-2 and fibroblasts or peripheral blood lymphocytes were analysed for the expression of HL-A antigens. The hybrids express the antigens of the donor lymphocytes or fibroblasts as well as other HL-A specificities from D98/AH-2, a HeLa derivative on which no HL-A could be detected by direct cytotoxicity. Segregant hybrid clones could be used to determine HL-A haplotypes and define the linkage relationships between these haplotypes and the PGM3 alleles expressed in the hybrids. An analysis of the HL-A antigens on D98/AH-2 and other HeLa derivates was done using absorption and a modified cytotoxicity assay.

MeSH terms

  • Alleles
  • Cell Line
  • Clone Cells
  • Epitopes
  • Fibroblasts / immunology*
  • Genetic Linkage
  • HLA Antigens / analysis*
  • Haploidy
  • Histocompatibility Antigens / analysis*
  • Humans
  • Hybrid Cells / immunology*
  • Lymphocytes / immunology*

Substances

  • Epitopes
  • HLA Antigens
  • Histocompatibility Antigens