Preparation and properties of co-reticulated invertase supported by an ultrafiltration membrane

Biochem J. 1977 Oct 1;167(1):313-5. doi: 10.1042/bj1670313.

Abstract

Yeast invertase was co-reticulated with glutaraldehyde to bovine serum albumin to give a soluble bound enzyme that was immobilized as a tightly adhering layer on the active surface of an ultrafiltration membrane. The Michaelis constant and stability of this immobilized-enzyme system are compared with those of the enzyme either in the native form or immobilized as a dynamically formed gel layer on an ultrafiltration membrane, as previously described by us [Drioli, Gianfreda, Palescandolo & Scardi (1975) Biotechnol, Bioeng, 17, 1365-1367].

MeSH terms

  • Enzymes, Immobilized*
  • Kinetics
  • Membranes, Artificial*
  • Serum Albumin, Bovine
  • Sucrase*
  • Ultrafiltration

Substances

  • Enzymes, Immobilized
  • Membranes, Artificial
  • Serum Albumin, Bovine
  • Sucrase