Abstract
The intracellular levels of poly(ADP-ribose) in cultured mouse cells were increased in response to hyperthermic treatment (43 degrees C). When hyperthermia was combined with other stressful treatments such as with ethanol and/or an alkylating agent, a dramatic synergistic increase in polymer levels was observed. The effect of hyperthermia did not appear to be related to the presence of DNA strand breaks. A possible involvement of poly(ADP-ribose) metabolism in the general cellular response to environmental stress is suggested.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Transformation, Neoplastic
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Cells, Cultured
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DNA / metabolism
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Ethanol / pharmacology*
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Hot Temperature*
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Kinetics
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Methylnitronitrosoguanidine / pharmacology*
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Mice
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Mice, Inbred BALB C
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Nucleoside Diphosphate Sugars / metabolism*
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Poly Adenosine Diphosphate Ribose / metabolism*
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Simian virus 40 / genetics
Substances
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Nucleoside Diphosphate Sugars
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Methylnitronitrosoguanidine
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Poly Adenosine Diphosphate Ribose
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Ethanol
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DNA