Binding of bacterial endotoxin (LPS) to encephalitogenic myelin basic protein and modulation of characteristic biologic activities of LPS

J Immunol. 1981 Mar;126(3):1030-5.

Abstract

Myelin basic protein, isolated from central nervous system tissue and an inducer of experimental allergic encephalomyelitis in animals, has been demonstrated to form a stable molecular complex with the lipid A region of gram-negative bacterial lipopolysaccharides (endotoxins). This binding of endotoxin with myelin basic protein results in generation of lower m.w. aggregates with decreased isopycnic density. A number of lipid A-induced characteristic properties of endotoxin, such as B lymphocyte proliferative response in C3H/St mice, complement activation of normal human serum, Limulus lysate gelation, and lethal effects in mice, are modified as a result of binding of myelin basic protein with lipopolysaccharides.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes
  • Binding Sites
  • Centrifugation, Isopycnic
  • Chromatography, Gel
  • Complement Activation
  • Female
  • Humans
  • Lipid A / pharmacology
  • Lipopolysaccharides / metabolism*
  • Lipopolysaccharides / physiology*
  • Lymphocyte Activation
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mortality
  • Myelin Basic Protein / metabolism*
  • Neutropenia / etiology
  • Rabbits

Substances

  • Lipid A
  • Lipopolysaccharides
  • Myelin Basic Protein