Pancreatic secretory factor (PSF), a protein from Gila monster venom stimulating enzyme secretion from rat pancreatic acini

FEBS Lett. 1984 Jan 30;166(2):283-7. doi: 10.1016/0014-5793(84)80096-4.

Abstract

Pancreatic secretory factor (PSF), a 17.5-kDa protein purified from the venom of Gila monster (Heloderma suspectum), stimulated amylase secretion from dispersed rat pancreatic acini more efficiently than CCK-8, bombesin, carbachol and secretin, and without increasing 45Ca2+ efflux and cyclic AMP levels. The secretory action was dependent on the presence of extracellular calcium and was additive to the secretion induced by agents acting via cyclic AMP or via Ca2+ efflux.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amylases / metabolism*
  • Animals
  • Biological Assay
  • Bombesin / pharmacology
  • Cyclic AMP / metabolism
  • Kinetics
  • L-Lactate Dehydrogenase / metabolism
  • Lizards
  • Pancreatic Juice / drug effects
  • Pancreatic Juice / enzymology*
  • Peptides / pharmacology*
  • Rats
  • Secretin / pharmacology
  • Sincalide / pharmacology
  • Vasoactive Intestinal Peptide / pharmacology
  • Venoms / analysis
  • Venoms / pharmacology*

Substances

  • Peptides
  • Venoms
  • pancreatic secretory factor, Gila monster venom
  • Secretin
  • Vasoactive Intestinal Peptide
  • Cyclic AMP
  • L-Lactate Dehydrogenase
  • Amylases
  • Sincalide
  • Bombesin