A routine method for contrasting elastin at the ultrastructural level

J Histochem Cytochem. 1984 Feb;32(2):251-8. doi: 10.1177/32.2.6198356.

Abstract

A reliable, simple, and inexpensive method for ultrastructural investigation of elastin is described. This method uses uranyl acetate dissolved in absolute methanol, followed by an optional lead citrate counterstain. The procedure was tested on a number of animal and human tissues that had been fixed and processed differently.

MeSH terms

  • Animals
  • Aorta / analysis
  • Cartilage / analysis
  • Cats
  • Elastin / analysis*
  • Gingiva / analysis
  • Guinea Pigs
  • Humans
  • Liver / analysis
  • Lung / analysis
  • Mesentery / analysis
  • Mice
  • Microscopy, Electron*
  • Organometallic Compounds*
  • Rats
  • Skin / analysis
  • Staining and Labeling*
  • Uranium*

Substances

  • Organometallic Compounds
  • uranyl acetate
  • Uranium
  • Elastin