The transience of cerebrocerebellar projections is due to selective elimination of axon collaterals and not neuronal death

Brain Res. 1984 Nov;318(2):301-6. doi: 10.1016/0165-3806(84)90034-8.

Abstract

Fluorescent dyes were used to determine firstly if the transience of cerebrocerebellar projections in neonatal kittens is due to the selective elimination of axon collaterals or to neuronal death; and secondly, if the cerebrocerebellar projection neurons lived, did any maintain a projection to the brainstem or spinal cord. Injections of Fast Blue were made into the cerebellar cortex and deep nuclei in 7-9 postnatal days old kittens, the age in which cortical axons grow into the cerebellum. Later, at 31-71 postnatal days of age, when the transient cerebrocerebellar projections have disappeared, injections of Nuclear Yellow were made into the brainstem or the spinal cord. In the frontoparietal cortex, numerous neurons were labeled with Fast Blue suggesting that the disappearance of cerebrocerebellar projections is due primarily to the selective elimination of axon collaterals and not neuronal death. Moreover, many of the cortical neurons labeled with Fast Blue also were labeled with Nuclear Yellow which shows that many of the cortical neurons with transient collateral projections to the cerebellum in the neonate maintain a projection to brainstem or spinal targets in older animals.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Axons / physiology
  • Cats
  • Cell Survival
  • Cerebellar Cortex / growth & development
  • Cerebellar Nuclei / growth & development
  • Cerebellum / growth & development*
  • Cerebral Cortex / growth & development*
  • Neural Pathways / growth & development
  • Pons / growth & development
  • Spinal Cord / growth & development
  • Staining and Labeling / methods