Synthesis and angiotensin-converting enzyme inhibitory activity of 3-(Mercaptomethyl)-2-oxo-1-pyrrolidineacetic acids and 3-(Mercaptomethyl)-2-oxo-1-piperidineacetic acids

J Med Chem. 1981 Jan;24(1):104-9. doi: 10.1021/jm00133a021.

Abstract

A number of gamma- and delta-lactam derivatives were synthesized and their in vitro angiotensin-converting enzyme (ACE) inhibitory activities were compared. The structures of these compounds were designed to include many of the important features of captopril. The synthesis involved the preparation of a variety of novel 3-methylene-2-pyrrolidinones (3-5 and 16) and 3-methylene-2-piperidinones (3a-5a, 10-12, and 17). The key intermediate 3-methylenelactams 3 and 3a were obtained from 3-(hydroxymethyl)lactams 2 and 2a by a direct dehydration with dicyclohexylcarbodiimide using cuprous iodide as a catalyst. Introduction of the sulfhydryl group was accomplished by a Michael addition of these alpha, beta-unsaturated lactams. The compound with the highest in vitro activity was 3-(mercaptomethyl)-2-oxo-1-piperidineacetic acid (7a). The activity of the 7a both in vitro and in vivo (dog) was shown to be less than that of captopril by a factor of about 100.

MeSH terms

  • Angiotensin-Converting Enzyme Inhibitors*
  • Animals
  • Captopril / pharmacology
  • Chemical Phenomena
  • Chemistry
  • Dogs
  • Guinea Pigs
  • Hemodynamics / drug effects
  • In Vitro Techniques
  • Muscle Contraction / drug effects
  • Pyrrolidinones / chemical synthesis*
  • Pyrrolidonecarboxylic Acid / analogs & derivatives
  • Pyrrolidonecarboxylic Acid / chemical synthesis*
  • Pyrrolidonecarboxylic Acid / pharmacology

Substances

  • Angiotensin-Converting Enzyme Inhibitors
  • Pyrrolidinones
  • Captopril
  • Pyrrolidonecarboxylic Acid