Abstract
We show that a mouse uterus nuclear phosphatase exists that is capable of inactivating nuclear oestrogen receptor complexed to oestradiol-17 beta in vitro but is ineffective when the receptor is complexed with the two non-steroidal anti-oestrogens, nafoxidine and tamoxifen. We suggest that the long half-life of the tamoxifen-receptor complex versus the short half-life of the oestradiol-receptor complex in uterine nuclei in vivo is the result of the ineffectiveness of the phosphatase in dephosphorylating the anti-oestrogen-receptor complex.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cattle
-
Cell Nucleus / drug effects
-
Cell Nucleus / enzymology
-
Cell Nucleus / metabolism
-
Cytosol / metabolism
-
Estradiol / metabolism
-
Female
-
Half-Life
-
In Vitro Techniques
-
Mice
-
Nafoxidine / pharmacology*
-
Phosphoric Monoester Hydrolases / metabolism
-
Pyrrolidines / pharmacology*
-
Receptors, Estradiol
-
Receptors, Estrogen / drug effects*
-
Tamoxifen / pharmacology*
-
Uterus / drug effects
-
Uterus / metabolism*
Substances
-
Pyrrolidines
-
Receptors, Estradiol
-
Receptors, Estrogen
-
Tamoxifen
-
Nafoxidine
-
Estradiol
-
Phosphoric Monoester Hydrolases