Abstract
A comparison has been made of the ability of vasoactive intestinal peptide (VIP), secretin, secretin analogues, and secretin-(7-27) to stimulate adenylate cyclase in rat pancreatic plasma membranes. A parallel study of the capacity of peptides of the VIP-secretin family to compete with 125I-VIP for binding to the same plasma membranes was conducted. This allowed a classification of VIP-secretin receptors into three subtypes: (1) VIP-preferring receptors; (2) high-affinity secretin receptors, and (3) low-affinity secretin receptors. The properties of secretin at high-affinity secretin receptors were likely to reflect a contribution of membranes from centroacinar and duct cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenylyl Cyclases / metabolism
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Animals
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Binding, Competitive
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Cell Membrane / analysis
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Dose-Response Relationship, Drug
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Gastrointestinal Hormones / analysis*
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Iodine Radioisotopes
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Pancreas / analysis*
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Rats
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Rats, Inbred Strains
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Receptors, Cell Surface / analysis*
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Receptors, G-Protein-Coupled
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Receptors, Gastrointestinal Hormone*
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Receptors, Vasoactive Intestinal Peptide
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Secretin / analogs & derivatives
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Secretin / analysis*
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Stimulation, Chemical
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Vasoactive Intestinal Peptide / analysis*
Substances
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Gastrointestinal Hormones
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Iodine Radioisotopes
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Receptors, Cell Surface
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Receptors, G-Protein-Coupled
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Receptors, Gastrointestinal Hormone
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Receptors, Vasoactive Intestinal Peptide
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secretin receptor
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Secretin
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Vasoactive Intestinal Peptide
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Adenylyl Cyclases