Abstract
PDGF is a potent mitogen that initiates the proliferation of quiescent fibroblastic cells. EGF and somatomedin C (or insulin) can replace the requirement for plasma to function synergistically with PDGF to stimulate DNA synthesis. PDGF, EGF and somatomedin C control discrete cellular events in the cell cycle. Cyclic AMP can potentiate the effects of polypeptide mitogens. The down-regulation of EGF receptors by PDGF and cyclic AMP brings about a loss of the requirement for exogenous EGF. The transient treatment of density-arrested fibroblasts with PDGF allows better study of synergistic actions of PDGF and plasma-derived factors. These synergistic interactions are important to understand in determining how multiple growth factors regulate cellular proliferation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Animals
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Blood
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Calcium / pharmacology
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Cell Cycle / drug effects*
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Cell Division / drug effects
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Cell Transformation, Neoplastic / drug effects
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Cell Transformation, Viral / drug effects
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Cells, Cultured
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Cyclic AMP / pharmacology
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DNA / biosynthesis
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Drug Synergism
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Epidermal Growth Factor / pharmacology
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ErbB Receptors
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Fibroblast Growth Factors / pharmacology
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Fibroblasts / cytology
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Growth Substances / pharmacology*
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Humans
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Insulin-Like Growth Factor I
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Mice
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Models, Biological*
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Platelet-Derived Growth Factor / pharmacology
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Protein Biosynthesis
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Receptors, Cell Surface / metabolism
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Receptors, Platelet-Derived Growth Factor
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Somatomedins / pharmacology
Substances
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Growth Substances
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Platelet-Derived Growth Factor
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Receptors, Cell Surface
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Somatomedins
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Fibroblast Growth Factors
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Epidermal Growth Factor
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Insulin-Like Growth Factor I
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DNA
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Cyclic AMP
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ErbB Receptors
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Receptors, Platelet-Derived Growth Factor
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Calcium