Abstract
Human hepatocellular carcinoma cells (Hep G2) were shown to secrete apo A-I as a proprotein . No apo A-I synthesis could be detected with endothelial cells from human umbilical cord veins. Conversion of proapo A-I into apo A-I is a slow (of the order of hours) process, mediated by a Ca2+/Mg2+-dependent enzyme which is present on the surface of plasma lipoprotein particles, endothelial cells and Hep G2 cells, and is probably synthesized by Hep G2 cells.
MeSH terms
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Apolipoprotein A-I
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Apolipoproteins / metabolism*
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Apolipoproteins A*
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Blood
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Calcium / metabolism
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Carcinoma, Hepatocellular / enzymology*
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Cell Line
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Endothelium / enzymology
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Female
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Humans
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Isoelectric Focusing
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Lipoproteins / metabolism
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Liver Neoplasms
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Magnesium / metabolism
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Molecular Weight
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Pregnancy
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Protein Precursors*
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Umbilical Veins / cytology
Substances
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Apolipoprotein A-I
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Apolipoproteins
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Apolipoproteins A
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Lipoproteins
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Protein Precursors
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Magnesium
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Calcium